Live demo · synthetic standard-curve assay
This is what “understand the experiment first” looks like.
No signup. AssayPilot detected an explicit standard-curve workflow, fitted the standards, back-calculated unknowns, applied the supplied dilution factor and kept QC visible.
Model4PL
R²1.000
Unknowns2
QC
PASSCalibration fit
4pl · R² = 1.000
PASSReplicates
0 sample group(s) above 20% CV
Why this analysis?
Standards, concentrations and assay signal were detected. Auto-selected 4pl by lower AICc (-547.92 vs -10.49).
Calibration model choice is based on the supplied standards; inspect residuals and assay-specific validation before publication.
Calculated figure
Not a screenshot.
This plot was generated by the same deterministic engine used for uploaded runs.